The type I-E CRISPR-Cas system influences the acquisition of blaKPC-IncF plasmid in Klebsiella pneumonia

Autor: Ying Zhou, Yu Tang, Pan Fu, Dongxing Tian, Lianhua Yu, Yunkun Huang, Gang Li, Meng Li, Yong Wang, Zehua Yang, Xiaogang Xu, Zhe Yin, Dongsheng Zhou, Laurent Poirel, Xiaofei Jiang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Emerging Microbes and Infections, Vol 9, Iss 1, Pp 1011-1022 (2020)
Druh dokumentu: article
ISSN: 22221751
2222-1751
DOI: 10.1080/22221751.2020.1763209
Popis: ABSTRACTKlebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) have disseminated worldwide and emerged as major threats to public health. Of epidemiological significance, the international pandemic of KPC-KP is primarily associated with CG258 isolates and blaKPC-IncF plasmids. CRISPR-Cas system is an adaptive immune system that can hinder gene expansion driven by horizontal gene transfer. Because of blaKPC-IncF plasmids are favored by CG258 K. pneumoniae, it was of interest to examine the co-distribution of CRISPR and blaKPC-IncF plasmids in such isolates. We collected 459 clinical K. pneumoniae isolates in China and collected 203 global whole-genome sequences in GenBank to determine the prevalence of CRISPR-Cas systems. We observed that CRISPR-Cas system was significantly scarce in the CG258 lineage and blaKPC-positive isolates. Furthermore, the results of conjugation and plasmid stability assay fully demonstrated the CRIPSR-Cas system in K. pneumoniae could effectively hindered blaKPC-IncF plasmids invasion and existence. Notably, most blaKPC-IncF plasmids were also proved to be good targets of CRISPR owing to carry matched and functional protospacers and PAMs. Overall, our work suggests that type I-E CRISPR-Cas systems could impact the spread of blaKPC in K. pneumoniae populations, and the scarcity of CRISPR-Cas system was one of potential factors leading to the propagation of blaKPC-IncF plasmids in CG258 K. pneumoniae.
Databáze: Directory of Open Access Journals
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