Microfluidic platform accelerates tissue processing into single cells for molecular analysis and primary culture models

Autor: Jeremy A. Lombardo, Marzieh Aliaghaei, Quy H. Nguyen, Kai Kessenbrock, Jered B. Haun
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-021-23238-1
Popis: Existing methods for tissue dissociation are inefficient and lead to variable outcomes and biases. Here, the authors present a microfluidic platform that combines digestion, disaggregation and filtration of tissue to allow single cell analysis and RNA sequencing.
Databáze: Directory of Open Access Journals