Panax Ginseng Extract Improves Follicular Development After Mouse Preantral Follicle 3D Culture

Autor: Abbas Majdi Seghinsara, Hamed Shoorei, Mohammad Mehdi Hassanzadeh Taher, Arash Khaki, Majid Shokooh, Moloud Tahmasebi, Amir Afshin Khaki, Hossein Eyni, Sadegh Ghorbani, Khadijeh Riahi Rad, Hossein Kalarestaghi, Leila Roshangar
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Cell Journal, Vol 21, Iss 2, Pp 210-219 (2019)
Druh dokumentu: article
ISSN: 2228-5806
2228-5814
DOI: 10.22074/cellj.2019.5733
Popis: Objective Panax ginseng is a popular traditional herb that has been used in complementary and alternative medicine in eastern Asia, and it possesses pharmacologically active compounds like ginsenosides (GSs). This study aimed to investigate the impact of Panax ginseng extract (PGE) at different concentrations on in vitro follicular function and development in a three-dimensional (3D) culture system fabricated using sodium alginate after 12 days of culture. Materials And Methods In this experimental study, preantral follicles (n=661) were mechanically isolated from the ovaries of 14-day-old female NMRI mice using 29-gauge insulin syringes. Follicles were individually capsulated within sodium alginate, and divided into four groups including control and experimental groups 1, 2, and 3. Then, they were cultured for 12 days in the medium supplemented with different concentrations of PGE (0, 50, 100, and 500 µg/ mL, for control groups and groups 1, 2 and 3, respectively). At the end of the culture period, the mean diameter and maturation of follicles, follicular steroid production, mRNA expression level of proliferating cell nuclear antigen (PCNA) and follicle stimulating hormone receptor (FSH-R), and reactive oxygen species (ROS) levels in collected metaphase-II (MII) oocytes were determined. Results The mean diameter of follicles in group 2 was significantly increased as compared to other groups (P
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