Chemical Composition and Antioxidant Activity of the Poll Gland Secretions of the Bactrian Camel (Camelus bactrianus)
| Autor: | Batjargal Batdorj, Munkh-Erdene Purev, Tserenjadamba Bayandorj, Damdindorj Lkhagvasuren, Oyunsuren Tsendsuren |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Central Asian Journal of Medical Sciences, Vol 3, Iss 2, Pp 132-139 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2413-8681 2414-9772 |
| DOI: | 10.24079/cajms.2017.06.005 |
| Popis: | Objectives: To determine chemical composition and antioxidant activity of poll gland secretions. Methods: Physical and chemical constituents, such as moisture and ash content, were determined using standard methods, crude fat was determined using the Soxhlet method, and organic composition was analyzed using GC-MS. Macro- and micro- element contents were determined using ICP-OES. Protein concentration was determined using the Bradford assay, and protein profiles were determined using SDS-PAGE. Antioxidant activity was determined using the DPPH free-radical scavenging technique. Results: Camel poll gland secretions contained 23 minerals. Potassium (89030 mg/kg) was most predominant, followed by calcium (12955 mg/kg), iron (8546 g/kg), aluminum (6105 mg/kg), magnesium (4563 mg/kg), sodium (2452 mg/kg), and zinc (14198 mg/kg). Total protein content was 11.7%. SDS-PAGE analysis of poll gland secretions revealed the presence of three different protein bands with the molecular weights of 35kDa, 68kDa and 130.5kDa. 12 organic compounds were determined by GC-MS in the poll gland secretions. Antioxidant activity of poll gland secretions was IC 50-176.2 µg/ ml. Conclusion: Proteins with the molecular weights of 35kDa, 68kDa, and 130.5kDa were determined in poll gland secretions, and they displayed potent antioxidant activity. |
| Databáze: | Directory of Open Access Journals |
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