The Study of HLA-G Gene and Protein Expression in Patients withRecurrent Miscarriage

Autor: Elnaz Mosaferi, Nazila Alizadeh Gharamaleki, Laya Farzadi, Jafar Majidi, Zohreh Babaloo, Tohid kazemi, Mehrnoosh Ramezani, Meraj Tabatabaei, Hamid Ahmadi, Leili Aghebati maleki, Behzad Baradaran
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Advanced Pharmaceutical Bulletin, Vol 9, Iss 1, Pp 70-75 (2019)
Druh dokumentu: article
ISSN: 2228-5881
2251-7308
DOI: 10.15171/apb.2019.009
Popis: Purpose: Although it has been frequently confirmed that HLA-G plays an important role in thereproduction and pregnancy, the pattern of HLA-G gene and its protein expression are rarelyaddressed in studies. Therefore we conducted this study in regard to evaluate the HLA-G geneand its protein expression in the women’s placenta with recurrent miscarriage.Methods: Placental samples were obtained from the women who were admitted for deliveryor abortion in Al Zahra and Taleghani hospitals, Tabriz, Iran. HLA-G gene expression wasdetermined by real-time polymerase chain reaction (PCR) and HLA-G protein expression wasassessed by western blotting and immunofluorescence staining in the tissue samples.Results: The results showed a significant decrease in the expression of gene and proteins ofHLA-G in the women with recurrent miscarriage compared to the control placental tissues.Conclusion: According to the obtained results, it was concluded that the decrement of HLA-Ggene and protein expressions are associated with recurrent miscarriage. Since there areconflicting results from other studies, it is suggested to conduct a more comprehensive similarstudy with greater sample size.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje