| Autor: |
Alina Fudulu, Marinela Bostan, Iulia Virginia Iancu, Adriana Pleșa, Adrian Albulescu, Irina Liviana Stoian, Demetra Gabriela Socolov, Gabriela Anton, Anca Botezatu |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Reports, Vol 7, Iss 3, p 71 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2571-841X |
| DOI: |
10.3390/reports7030071 |
| Popis: |
(1) Background: Cervical cancer, caused mainly by high-risk Human Papillomavirus (hrHPV), is a significant global health issue. While a Pap smear remains a reliable method for early detection, identifying new biomarkers to stratify the risk is crucial. For this purpose, extensive research has been conducted on detecting DNA methylation. (2) Methods: This cross-sectional study aimed to assess the expression levels of EIF4G3 and SF3B1 in precursor lesions and cervical tumor tissues through qRT-PCR and evaluate the methylation status of their promoters through bisulfite conversion. (3) Results: Both genes showed similar mRNA expression patterns, with the highest levels observed in squamous cell carcinoma (SCC) samples (p < 0.0001). Additionally, methylation analysis indicated increased percentages in the control group for both factors. Notably, the expression levels of both genes were inversely correlated with promoter methylation (EIF4G3—p = 0.0016; SF3B1—p < 0.0001). (4) Conclusions: Regarding the methylation pattern for both genes, we observe a decreasing trend from NILM to SCC patients. Therefore, we concluded that the decrease in methylation at the promoter level for both genes could be an indicator of abnormal cytology. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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