Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR

Autor: ZOU Lin, JIA Nan, ZHANG Ping, ZHEN Bojun, GUO Xiaochen, WANG Fang, JI Guoqiang, YAN Aixia, KANG Ying, MA Hongmei, LI Ying
Jazyk: čínština
Rok vydání: 2024
Předmět:
Zdroj: Zhongguo shipin weisheng zazhi, Vol 36, Iss 4, Pp 389-393 (2024)
Druh dokumentu: article
ISSN: 1004-8456
DOI: 10.13590/j.cjfh.2024.04.004
Popis: ObjectiveTo lay the foundation of quickly and effectively response to such epidemics for laboratories, the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.MethodsBiological samples from the 3 outbreaks of Campylobacter were collected. Bacterial culture was performed on the samples using filter culture method. DNA was extracted from the original samples, 24 h enriched samples and 48 h enriched samples. Kappa test was used to analyze the results of real-time PCR and culture method.ResultsThe real-time PCR detection sensitivity of original samples, 24 h enriched samples and 48 h enriched samples were 90.91%, 97.22% and 100%. And the detection specificity were 75.00%, 84.00%和78.95%, respectively. Kappa value for the consistency analysis in culture method with real-time PCR based on original samples, 24 h enriched culture samples and 48 h enriched samples were 0.643, 0.813 and 0.785, respectively.ConclusionThe combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.
Databáze: Directory of Open Access Journals