| Autor: |
Nazanin‐Zahra Shafiei‐Jandaghi, Forough Tavakoli, Jila Yavarian, Vahid Salimi, Kaveh Sadeghi, Sevrin Zadheidar, Ahmad Nejati, Somayeh Shatizadeh Malekshahi, Talat Mokhtari‐Azad |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Health Science Reports, Vol 6, Iss 9, Pp n/a-n/a (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2398-8835 |
| DOI: |
10.1002/hsr2.1538 |
| Popis: |
Abstract Background and Aims Real‐time reverse‐transcriptase polymerase chain reaction (real‐time RT‐PCR) is the gold standard test for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). However, when the test result is near the detection limit of the assay the possibility of getting false positive or negative results is high. In addition, it might result in single target gene positive (STGP) results which should be interpreted with caution. Methods This study was performed on 29,962 nasal swabs from July 1 to August 31, 2020. Ct values less than 40 for each or both of N and RdRp genes were recommended to be selected as positive. Positive samples for one gene with the Cts more than 35 were rechecked by adding more templates. Results The results showed that 1016 (3.39%) samples were positive just for one gene with high Ct values. The results of the second reactions showed that 325 (31.99%) samples were positive for both N and RdRp which were reported positive, 301 (29.65%) were positive only for one gene which were considered as suspicious cases and resampling was suggested for them. Finally, 390 (38.385%) samples were negative for both genes. Conclusion In conclusion, tracking weak positive results of SARS‐CoV‐2 real‐time RT‐PCR revealed that most of the individuals who were STGP clean the infection completely in less than a week which showed they were in the convalescent phase of infection. However, some of them who were in the beginning of infection showed a decrease in Ct value during a week, so they could spread the virus in the society. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text