| Autor: |
Theresa Jox, Melanie K. Buxa, Dorte Bohla, Ikram Ullah, Igor Mačinković, Alexander Brehm, Marek Bartkuhn, Rainer Renkawitz |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Epigenetics & Chromatin, Vol 10, Iss 1, Pp 1-14 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
1756-8935 |
| DOI: |
10.1186/s13072-017-0140-6 |
| Popis: |
Abstract Background Chromatin insulators shield promoters and chromatin domains from neighboring enhancers or chromatin regions with opposing activities. Insulator-binding proteins and their cofactors mediate the boundary function. In general, covalent modification of proteins by the small ubiquitin-like modifier (SUMO) is an important mechanism to control the interaction of proteins within complexes. Results Here we addressed the impact of dSUMO in respect of insulator function, chromatin binding of insulator factors and formation of insulator speckles in Drosophila. SUMOylation augments the enhancer blocking function of four different insulator sequences and increases the genome-wide binding of the insulator cofactor CP190. Conclusions These results indicate that enhanced chromatin binding of SUMOylated CP190 causes fusion of insulator speckles, which may allow for more efficient insulation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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