| Autor: |
Shiau‐Chi Chen, Neng‐Jie Zeng, Grace Y. Liu, Hsien‐Chu Wang, Tzu‐Ying Lin, Yi‐Ling Tai, Chiao‐Yun Chen, Yin Fang, Yi‐Chien Chuang, Ching‐Lin Kao, Hsuan Cheng, Bing‐Huang Wu, Pin‐Chiao Sun, Odvogmed Bayansan, Yu‐Ting Chiu, Chi‐Hsuan Shih, Wen‐Hong Chung, Jia‐Bin Yang, Lily Hui‐Ching Wang, Po‐Han Chiang, Chun‐Hao Chen, Oliver I. Wagner, Yi‐Ching Wang, Yu‐Chun Lin |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Advanced Science, Vol 11, Iss 45, Pp n/a-n/a (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2198-3844 |
| DOI: |
10.1002/advs.202405568 |
| Popis: |
Abstract Intracellular trafficking, an extremely complex network, dynamically orchestrates nearly all cellular activities. A versatile method that enables the manipulation of target transport pathways with high spatiotemporal accuracy in vitro and in vivo is required to study how this network coordinates its functions. Here, a new method called RIVET (Rapid Immobilization of target Vesicles on Engaged Tracks) is presented. Utilizing inducible dimerization between target vesicles and selective cytoskeletons, RIVET can spatiotemporally halt numerous intracellular trafficking pathways within seconds in a reversible manner. Its highly specific perturbations allow for the real‐time dissection of the dynamic relationships among different trafficking pathways. Moreover, RIVET is capable of inhibiting receptor‐mediated endocytosis. This versatile system can be applied from the cellular level to whole organisms. RIVET opens up new avenues for studying intracellular trafficking under various physiological and pathological conditions and offers potential strategies for treating trafficking‐related disorders. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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