Autor: |
Yawen Song, Hui Sun, Kailiang Wu, Jianke Lyu, Jingyue Zhang, Feng Gu, Yongjie Ma, Beibei Shen, Chijuan Wang, Xiaojiao Chen, Jing Xu, Weidong Li, Fangfang Liu, Li Fu |
Jazyk: |
angličtina |
Rok vydání: |
2021 |
Předmět: |
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Zdroj: |
Cancer Biology & Medicine, Vol 18, Iss 2, Pp 477-489 (2021) |
Druh dokumentu: |
article |
ISSN: |
2095-3941 |
DOI: |
10.20892/j.issn.2095-3941.2020.0422 |
Popis: |
Objective: Mucin 1 (MUC1/EMA) and sialyl Lewis X (sLex) indicate polarity reversal in invasive micropapillary carcinoma (IMPC). The purpose of this study was to evaluate the expression of MUC1/EMA and sLex and to assess their diagnostic and prognostic value in patients with IMPC. Methods: The expression of sLex and MUC1/EMA in 100 patients with IMPC and a control group of 89 patients with invasive ductal carcinoma not otherwise specified (IDC-NOS) were analyzed with IHC. Fresh tumor tissues were collected from patients with IMPC or IDC-NOS for primary culture and immunofluorescence analysis. Results: The rate of nodal metastasis was higher in patients with IMPC than those with IDC-NOS, and IMPC cells tended to express more sLex and MUC1/EMA in the cytomembranes (the stroma-facing surfaces of the micropapillary clusters) than IDC-NOS cells. In IMPC, high cytomembrane expression of sLex, but not MUC1/EMA, indicated poor prognosis. In addition, among the 100 patients with IMPC, 10 patients had sLex+/EMA– expression patterns, and 8 patients had sLex–/EMA+ expression patterns. The primary IMPC cells were suspended, non-adherent tumor cell clusters, whereas the primary IDC cells were adherent tumor cells. Immunofluorescence analysis showed that MUC1/EMA and sLex were co-expressed on the cytomembranes in IMPC cell clusters and in the cytoplasm in IDC-NOS cells. Conclusions: sLex can be used as a prognostic indicator and can be combined with MUC1/EMA as a complementary diagnostic indicator to avoid missed IMPC diagnosis. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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