Quantitative micro determination and isolation of plasmalogen aldehydes as 2,4-dinitrophenylhydrazones

Autor:	Ira Katz, Mark Keeney
Jazyk:	angličtina
Rok vydání:	1966
Předmět:	plasmalogen aldehydes determination 2 4-dinitrophenylhydrazones spectrophotometry thin-layer chromatography aldehyde Biochemistry QD415-436
Zdroj:	Journal of Lipid Research, Vol 7, Iss 1, Pp 170-174 (1966)
Druh dokumentu:	article
ISSN:	0022-2275
DOI:	10.1016/S0022-2275(20)39602-4
Popis:	A micro spectrophotometric procedure for the quantitative determination of plasmalogen aldehydes is described which utilizes simultaneous methanolysis and formation of 2,4-dinitrophenylhydrazones. After isolation of the hydrazones by thin-layer chromatography, the aldehydes can be regenerated, reduced, acetylated, and then analyzed by gas–liquid chromatography. Identification of the plasmalogen aldehydes obtained from rumen holotrich protozoa is described.
Databáze:	Directory of Open Access Journals
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