| Autor: |
Akira Uchida, Hie Lim Kim, Rikky W. Purbojati, Vineeth Kodengil Vettath, Anjali B. Gupta, James N. I. Houghton, Caroline Chénard, Anthony Wong, Megan E. Clare, Kavita K. Kushwaha, Deepa Panicker, Alexander Putra, Cassie E. Heinle, Balakrishnan N. V. Premkrishnan, Ana Carolina M. Junqueira, Daniela I. Drautz-Moses, Stephan C. Schuster |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Gut Pathogens, Vol 12, Iss 1, Pp 1-7 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1757-4749 |
| DOI: |
10.1186/s13099-020-00350-z |
| Popis: |
Abstract Background Enterobacter cloacae complex (ECC) bacteria, such as E. cloacae, E. sichuanensis, E. kobei, and E. roggenkampii, have been emerging as nosocomial pathogens. Many strains isolated from medical clinics were found to be resistant to antibiotics, and in the worst cases, acquired multidrug resistance. We present the whole genome sequence of SGAir0282, isolated from the outdoor air in Singapore, and its relevance to other ECC bacteria by in silico genomic analysis. Results Complete genome assembly of E. sichuanensis strain SGAir0282 was generated using PacBio RSII and Illumina MiSeq platforms, and the datasets were used for de novo assembly using Hierarchical Genome Assembly Process (HGAP) and error corrected with Pilon. The genome assembly consisted of a single contig of 4.71 Mb and with a G+C content of 55.5%. No plasmid was detected in the assembly. The genome contained 4371 coding genes, 83 tRNA and 25 rRNA genes, as predicted by NCBI’s Prokaryotic Genome Annotation Pipeline (PGAP). Among the genes, the antibiotic resistance related genes were included: Streptothricin acetdyltransferase (SatA), fosfomycin resistance protein (FosA) and metal-dependent hydrolases of the beta-lactamase superfamily I (BLI). Conclusion Based on whole genome alignment and phylogenetic analysis, the strain SGAir0282 was identified to be Enterobacter sichuanensis. The strain possesses gene clusters for virulence, disease and defence, that can also be found in other multidrug resistant ECC type strains. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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