Synergistic interaction of eugenol and antimicrobial drugs in eradication of single and mixed biofilms of Candida albicans and Streptococcus mutans

Autor:	Huma Jafri, Gopa Banerjee, Mohd Sajjad Ahmad Khan, Iqbal Ahmad, Hussein Hasan Abulreesh, Abdullah Safar Althubiani
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	C. albicans S. mutans SMIC PMIC Biofilm eradication FICI Biotechnology TP248.13-248.65 Microbiology QR1-502
Zdroj:	AMB Express, Vol 10, Iss 1, Pp 1-9 (2020)
Druh dokumentu:	article
ISSN:	2191-0855
DOI:	10.1186/s13568-020-01123-2
Popis:	Abstract In vitro eradication of the C. albicans and S. mutans mixed biofilms by eugenol alone and in combination with the antimicrobial drugs. Previously characterized strains of C. albicans (CAJ-01 and CAJ-12) and S. mutans MTCC497 were used to evaluate the eradication of biofilms using XTT reduction assay, viability assay, time dependent killing assay and scanning electron microscopy (SEM). Synergistic interaction was assessed by checkerboard method. Sessile MIC (SMIC) of eugenol was equivalent to the planktonic MIC (PMIC) against C. albicans and S. mutans mixed biofilms. SMIC of fluconazole and azithromycin was increased upto 1000-folds over PMIC. Eradication of single or mixed biofilms was evident from the viability assay and SEM. At 1 × MIC of eugenol, log10CFU count of C. albicans cells were decreased from 6.3 to 4.2 and 3.8 (p
Databáze:	Directory of Open Access Journals
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