Autor: |
Gisele V. Baracho, Nihan Kara, Stephanie Rigaud, Evelyn Lo, Stephanie J. Widmann, Aaron J. Tyznik |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 3, Iss 1, Pp 101069- (2022) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2021.101069 |
Popis: |
Summary: Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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