Cell Therapy with Human Reprogrammed CD8+ T-Cells Has Antimetastatic Effects on Lewis Lung Carcinoma in C57BL/6 Mice

Autor: Evgenii G. Skurikhin, Olga Pershina, Natalia Ermakova, Angelina Pakhomova, Mariia Zhukova, Edgar Pan, Lubov Sandrikina, Darius Widera, Lena Kogai, Nikolai Kushlinskii, Aslan Kubatiev, Sergey G. Morozov, Alexander Dygai
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Molecular Sciences, Vol 23, Iss 24, p 15780 (2022)
Druh dokumentu: article
ISSN: 23241578
1422-0067
1661-6596
DOI: 10.3390/ijms232415780
Popis: Using a model of Lewis lung carcinoma (LLC) in vitro and in vivo, we previously demonstrated increased antitumor activity in CD8+ T-cells reprogrammed with an MEK inhibitor and PD-1 blocker. In this follow-up study, we carried out the reprogramming of human CD8+ T-cells (hrT-cell) using the MEK inhibitor and PD-1 blocker and targeted LLC cells. The effects of hrT-cell therapy were studied in a mouse model of spontaneous metastasis of a solid LLC tumor. We found antimetastatic activity of hrT-cells, a decrease in the number of cancer cells and cancer stem cells in the lungs, and an increase in the number of T-cells in the blood (including effector T-cells). Thus, reprogramming of human CD8+ T-cells with an MEK inhibitor and PD-1 blocker with targeted training by tumor target cells is a potential platform for developing a new approach to targeted lung cancer therapy.
Databáze: Directory of Open Access Journals
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