The endometrial expression level of estrogen-α and VEGF-A in female rats administered with a low dose of estrogen in early clomiphene citrate therapy
| Autor: | Rahmat L. Landahur, Budi S. Santoso, Widjiati Widjiati, Epy M. Luqman |
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| Jazyk: | Arabic<br />English |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Iraqi Journal of Veterinary Sciences, Vol 37, Iss 1, Pp 223-228 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1607-3894 2071-1255 |
| DOI: | 10.33899/ijvs.2022.133333.2207 |
| Popis: | The study was conducted to determine the effect of low-dose estrogen in the early cycle of clomiphene citrate therapy on the expression of level estrogen receptor α (ERα) and VEGF-A in the endometrium. This study was an experimental study using 29 adult female rats aged 3 months with the body weight of 150-200 grams. The female rats were randomly allocated were allocated randomly into 3 groups (C: placebo; T1: treated with a single dose of clomiphene citrate (100 mg/kg BW, IP, single dose); T2: combination treatment with clomiphene citrate (100 mg/kg BW, IP, single dose) and estradiol valerat (15 pg/kg BW, IP, single dose). Uterine samples were examined for estrogen-α receptor expression and VEGF-A expression using immunohistochemistry. The results revealed a significant difference between the percentage of Estrogen-α in the C group compared with the T1 and T2 groups. Meanwhile, there was no significant difference between the T1 and T2 groups. The percentage of VEGF-A expression in group C was significantly different compared with T1 and T2. Meanwhile, there was no significant difference between the T1 and T2 groups. In conclusion, administration of a low dose of estradiol valeratein early clomiphene citrate therapy resulted in no significant effect on estrogen-α receptor expression and VEGF-A expression in the endometrium. |
| Databáze: | Directory of Open Access Journals |
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