Isolation and characterization of glycoprotein (CNP) isolated from Cocos nucifera L. nutshell and its immunomodulatory role on macrophage activation

Autor:	Sujatha Srinivasan, Adithyan Jayaraman, Aishwarya Ramasamy, Kirubaa Mohanraj, Mamilla R. Charan Raja, Santanu Kar Mahapatra
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Cocos nucifera L. Nutshell protein Giberillin-20-oxidase Serine/threonine kinase Macrophage activation Immunomodulation Nutrition. Foods and food supply TX341-641
Zdroj:	Journal of Functional Foods, Vol 100, Iss , Pp 105380- (2023)
Druh dokumentu:	article
ISSN:	1756-4646
DOI:	10.1016/j.jff.2022.105380
Popis:	The crude protein from Cocos nucifera L. nutshell was isolated and fractionized by GFC, yielding two protein peaks, CNP-F1 and CNP-F2 having a molecular weight of 43 kDa and 46 kDa, respectively as confirmed by MALDI-TOF. MS/MS analysis followed by MASCOT search confirmed CNP-F1 was gibberellin-20-oxidase and CNP-F2 was serine/threonine kinase-11. CNP-F1 (2.5 µg/mL) and CNP-F2 (0.5 µg/mL) upregulated the pro-inflammatory cytokines (IL-12, IFN-γ) release significantly (p
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/cf233914c434445aa8878b4ab29cc3f3 Zobrazit plný text záznamu Full Text from ScienceDirect View record in DOAJ