Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings
| Autor: | Jonas Michel Wolf, Tatiana Schäffer Gregianini, Claudete Maria Farina Seadi, Gabriela Luchiari Tumioto, Bibiana Paula Dambrós, Fernanda Kieling Moreira Lehmann, Silvia De Carli, Nilo Ikuta, Vagner Ricardo Lunge |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 6, Pp 762-764 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1678-9849 0037-8682 |
| DOI: | 10.1590/0037-8682-0107-2015 |
| Popis: | ABSTRACT INTRODUCTION: Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) to detect hMPV in a clinical laboratory setting. METHODS: Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays. RESULTS: In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofluorescence specificity was 99% and sensitivity was 38%. CONCLUSIONS: DIF is not very sensitive under clinical laboratory settings. |
| Databáze: | Directory of Open Access Journals |
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