Autor: |
Lisa Welter, Serena Zheng, Sonia Maryam Setayesh, Michael Morikado, Arushi Agrawal, Rafael Nevarez, Amin Naghdloo, Milind Pore, Nikki Higa, Anand Kolatkar, Jana-Aletta Thiele, Priyanka Sharma, Halle C. F. Moore, Jennifer K. Richer, Anthony Elias, Kenneth J. Pienta, Amado J. Zurita, Mitchell E. Gross, Stephanie N. Shishido, James Hicks, Carmen Ruiz Velasco, Peter Kuhn |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
Cancers, Vol 15, Iss 15, p 3949 (2023) |
Druh dokumentu: |
article |
ISSN: |
2072-6694 |
DOI: |
10.3390/cancers15153949 |
Popis: |
Bi-directional crosstalk between the tumor and the tumor microenvironment (TME) has been shown to increase the rate of tumor evolution and to play a key role in neoplastic progression, therapeutic resistance, and a patient’s overall survival. Here, we set out to use a comprehensive liquid-biopsy analysis to study cancer and specific TME cells in circulation and their association with disease status. Cytokeratin+, CD45- circulating rare cells (CRCs) from nine breast and four prostate cancer patients were characterized through morphometrics, single-cell copy number analysis, and targeted multiplexed proteomics to delineate cancer cell lineage from other rare cells originating in the TME. We show that we can detect epithelial circulating tumor cells (EPI.CTC), CTCs undergoing epithelial-to-mesenchymal transition (EMT.CTC) and circulating endothelial cells (CECs) using a universal rare event detection platform (HDSCA). Longitudinal analysis of an index patient finds that CTCs are present at the time of disease progression, while CECs are predominately present at the time of stable disease. In a small cohort of prostate and breast cancer patients, we find high inter-patient and temporal intra-patient variability in the expression of tissue specific markers such as ER, HER2, AR, PSA and PSMA and EpCAM. Our study stresses the importance of the multi-omic characterization of circulating rare cells in patients with breast and prostate carcinomas, specifically highlighting overlapping and cell type defining proteo-genomic characteristics of CTCs and CECs. |
Databáze: |
Directory of Open Access Journals |
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