Homoharringtonine promotes heart allograft acceptance by enhancing regulatory T cells induction in a mouse model
| Autor: | Xia Qiu, Hedong Zhang, Zhouqi Tang, Yuxi Fan, Wenjia Yuan, Chen Feng, Chao Chen, Pengcheng Cui, Yan Cui, Zhongquan Qi, Tengfang Li, Yuexing Zhu, Liming Xie, Fenghua Peng, Tuo Deng, Xin Jiang, Longkai Peng, Helong Dai, Yuanyuan Ji |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Chinese Medical Journal, Vol 137, Iss 12, Pp 1453-1464 (2024) |
| Druh dokumentu: | article |
| ISSN: | 0366-6999 2542-5641 00000000 |
| DOI: | 10.1097/CM9.0000000000002813 |
| Popis: | Abstract. Background:. Homoharringtonine (HHT) is an effective anti-inflammatory, anti-viral, and anti-tumor protein synthesis inhibitor that has been applied clinically. Here, we explored the therapeutic effects of HHT in a mouse heart transplant model. Methods:. Healthy C57BL/6 mice were used to observe the toxicity of HHT in the liver, kidney, and hematology. A mouse heart transplantation model was constructed, and the potential mechanism of HHT prolonging allograft survival was evaluated using Kaplan–Meier analysis, immunostaining, and bulk RNA sequencing analysis. The HHT-T cell crosstalk was modeled ex vivo to further verify the molecular mechanism of HHT-induced regulatory T cells (Tregs) differentiation. Results:. HHT inhibited the activation and proliferation of T cells and promoted their apoptosis ex vivo. Treatment of 0.5 mg/kg HHT for 10 days significantly prolonged the mean graft survival time of the allografts from 7 days to 48 days (P |
| Databáze: | Directory of Open Access Journals |
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