| Autor: |
Seema Rohilla, Rajendra Awasthi, Ankur Rohilla, Sachin Kumar Singh, Dinesh Kumar Chellappan, Kamal Dua, Harish Dureja |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
ADMET and DMPK (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1848-7718 |
| DOI: |
10.5599/admet.2366 |
| Popis: |
This study aimed to improve the stability and prolonged gefitinib release from the nanoliposomes. Nanoliposomes were prepared by reverse-phase evaporation and optimized using Box-Behnken design to investigate the influence of sonication time (X1), tween 80 / soya phosphatidylcholine ratio (X2), and cholesterol / soya phosphatidylcholine ratio (X3) on nanoliposomes. Optimized nanoliposomes were quasi-spherical shaped, with a mean dimension of 93.2 nm and an encapsulation efficiency of 87.56±0.17 %. Surface decoration of the optimized batch was done using different concentrations of chitosan. The optimal chitosan concentration required to adorn the surface of nanoliposomes was 0.01 %. In comparison to unadorned nanoliposomes (82.16±0.65 %), adorned nanoliposomes (78.04±0.35 %) released the drug consistently over 24 h via Fickian diffusion. The IC50 values for surface-adorned nanoliposomes in A549 and H1299 cells were 6.53±0.75 and 4.73±0.46 µM, respectively. Cytotoxicity of the surface-decorated nanoliposomes may be due to their higher zeta potential and prolonged drug release. At 4°C, adorned and unadorned nanoliposomes are most stable. In conclusion, the developed nanoliposomes may offer a new path for melanoma clinics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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