The Effect of miR-106b-5p Expression in The Production of iPS-Like Cells from Mice SSCs during The Formation of Teratoma and The Three Embryonic Layers
Autor: | Amir Hossein Hasani Fard, Fatemeh Kamalipour, Zohreh Mazaheri, Seyed Jalil Hosseini |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Cell Journal, Vol 24, Iss 8, Pp 442-448 (2022) |
Druh dokumentu: | article |
ISSN: | 2228-5806 2228-5814 |
DOI: | 10.22074/cellj.2022.8147 |
Popis: | Objective: According to the mounting data, microRNAs (miRNAs) may play a key role in reprogramming. miR-106bis considered as an enhancer in reprogramming efficiency. Based on induced pluripotent stem cells (iPSCs), cell treatments have a huge amount of potential. One of the main concerns about using iPSCs in therapeutic settings is the possibility of tumor formation. It is hypothesized that a procedure that can reprogram cells with less genetic manipulation reduces the possibility of tumorigenicity.Materials and Methods: In this experimental study, miR-106b-5p transduced by pLV-miRNA vector into mice isolated spermatogonial stem cells (SSCs) to achieve iPS-like cells. Then the transduced cells were cultured in specific conditions to study the formation of three germ layers. The tumorigenicity of these iPS-like cells was investigated by transplantation into male BALB/C mice.Results: We show that SSCs can be successfully reprogrammed into induced iPS-like cells by pLV-miRNA vector to transduce the hsa-mir-106b-5p into SSCs and generating osteogenic, neural and hepatoblast lineage cells in vitro as a result of pluripotency. Although these iPS-like cells are pluripotent, they cannot form palpable tumors in vivo.Conclusion: These results demonstrate that infection of hsa-mir-106b-5p into SSCs can reprogram them into iPSCsand advanced germ cell lineages without tumorigenicity. Also, a novel approach for studying the generation of iPSCsand the application of iPS or iPS-like cells in regenerative medicine is presented. |
Databáze: | Directory of Open Access Journals |
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