Modulation of Folate Dependent DNA Methyltransferase 1 (dnmt1) Activity in Whole Body γ-irradiated Mice.
| Autor: | Batra Vipen, Kesavan Vellapan, Mishra Kaushal P. |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: | |
| Zdroj: | Pteridines, Vol 15, Iss 4, Pp 155-160 (2004) |
| Druh dokumentu: | article |
| ISSN: | 0933-4807 2195-4720 |
| DOI: | 10.1515/pteridines.2004.15.4.155 |
| Popis: | The methylation pattern of DNA is established during embryogenesis and is subsequently maintained by maintenance methylation activity of the enzyme DNA methyltransferase 1 (dnmtl). The modulation of nuclear dnmtl activity in nuclear and post-nuclear supernatant fractions was studied after various time intervals in 1, 3. 5 and 7 Gy whole body γ-irradiated mice. Significant changes in enzyme activity were observed in nuclear fraction of liver at various stages after irradiation. Our results show a dose-dependent increase in nuclear dnmtl activity after 24 hours al 1 to 5 Gy, with the maximum increase in activity at 7 Gy. An attenuation of enzyme activity was observed after 48 hours, which was continued at 96 hours post-iradiation, where the activity approached subnormal levels at higher doses. The dnmtl activity was less conspicuous in supernatant fraction and no significant change in activity was observed 24 hours after irradiation. Present studies suggest the possible role of radiation as a stressor in modulating the folate dependent dnmtl activity, which in turn may affect global DNA methylation pattern. |
| Databáze: | Directory of Open Access Journals |
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