| Autor: |
Sugeng Winardi, Arief Widjaja, Nadiem Anwar |
| Jazyk: |
angličtina |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Makara Seri Sains, Vol 14, Iss 2, Pp 113-116 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
1693-6671 |
| Popis: |
Increasing the Performance of Enzymatic Hydrolysis of Rice Straw Using Mixed Crude Cellulases fromTrichoderma reesei and Aspergillus niger. The objective of this work is to compare the effectiveness of mixed crudeenzyme cellulase from T. reesei and A. niger with commercial enzyme from A. niger, and to investigate effect ofenzyme to substrate ratio to performance of enzymatic hydrolysis of rice straw. The commercial enzyme from FlukaBiochemica was used, and crude enzyme were prepared by solid fermentation with simple media. Before hydrolized,the rice straw was grinded and sieved and then heated at 85 oC with 2% sodium hydroxide for six hours. Hydrolysis wasconducted in 300 mL beaker flask equipped with mechanical stirrer. Samples were analyzed by dinitrosalicylic acidmethod and measured by spectrophotometer. Both of commercial and mixed crude enzyme show that, the higherenzyme to substrate ratio was higher the glucose concentration obtained. However, ratio of glucose obtained to enzymeused become smaller. The mixture of crude enzyme from T. reesesi dan A. niger that produced in this work was twofold more effective to hydrolyze rice straw than using cellulase enzyme of A. niger from Fluka Biochemika |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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