Autor: |
D Gurantz, M F Laker, A F Hofmann |
Jazyk: |
angličtina |
Rok vydání: |
1981 |
Předmět: |
|
Zdroj: |
Journal of Lipid Research, Vol 22, Iss 2, Pp 373-376 (1981) |
Druh dokumentu: |
article |
ISSN: |
0022-2275 |
DOI: |
10.1016/S0022-2275(20)35380-3 |
Popis: |
An enzymatic method, previously used to determine choline-containing phospholipids in serum, was applied to the estimation of phospholipid concentration in bile. The method combines three enzymatic reactions: a) release of choline by phospholipase D; b) choline oxidation by choline oxidase, a reaction which generates hydrogen peroxide; and c) formation of a red quinone dye by peroxidase. The method is an endpoint, spectrophotometric determination. It is simple, sensitive, and reproducible. Accuracy was demonstrated both by linear recoveries of the choline-containing phospholipids in bile and a good correlation with a chemical determination. No interferences by bile acids and/or cholesterol were observed; bile pigment interference was removed by bleaching with white light. This method which determines lipid choline is simpler than the widely used chemical determination of lipid phosphorus. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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