| Autor: |
Bo Liu, Lv Yang, Xiu-Juan Li, Rou Li, Wei Sun, Xiao-Yi Chen, Jun-Chao Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Kaohsiung Journal of Medical Sciences, Vol 34, Iss 6, Pp 321-329 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1607-551X |
| DOI: |
10.1016/j.kjms.2017.12.011 |
| Popis: |
To study the significance of apoptosis stimulating protein of P53 2 (ASPP2) expression in esophageal squamous cell carcinoma (ESCC), immunohistochemistry S-P method was used to examine the expression of ASPP2 in 136 cases of ESCC, 35 cases of high grade intraepithelial neoplasia (HGIN), 29 cases of low grade intraepithelial neoplasia (LGIN) and 37 cases of normal esophageal epithelium (NEE). The associations of ASPP2 expression with clinicopathological data and overall survival (OS) were also analyzed. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate ASPP2 expression in a total of 20 matched human ESCC tumor tissues and normal adjacent tissues (NAT). In addition, EC109 cells were treated with cisplatin (CDDP) in vitro for 24 h (the intervention group) and the control group was set up at the same time. Western blot was used to examine the expression of ASPP2 protein between the two groups. The expression of ASPP2 decreased progressively from NEE to LGIN, to HGIN, and to ESCC, and it was related to TNM stage, histological differentiation and lymph node metastasis in ESCC (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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