Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough?
| Autor: | M.J. Mimica, E.N. Berezin, R.L.B. Carvalho, I.M. Mimica, L.M.J. Mimica, M.A.P. Sáfadi, E. Schneider, H.H. Caiaffa-Filho |
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| Jazyk: | angličtina |
| Předmět: | |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 11, Iss 4, Pp 415-417 |
| Druh dokumentu: | article |
| ISSN: | 1678-4391 1413-8670 |
| DOI: | 10.1590/S1413-86702007000400009 |
| Popis: | We evaluated the performance of several methods for the detection of methicillin resistance in Staphylococcus aureus using 101 clinical S. aureus isolates from pediatric patients in a tertiary hospital in Brazil; 50 isolates were mecA-positive and 51 were mecA-negative. The Etest and oxacillin agar screening plates were 100% sensitive and specific for mecA presence. Oxacillin and cefoxitin disks gave sensitivities of 96 and 92%, respectively, and 98% specificity. Alterations of CLSI cefoxitin breakpoints increased sensitivity to 98%, without decreasing specificity. Our results highlight the importance of a continuing evaluation of the recommended microbiological methods by different laboratories and in different settings. If necessary, laboratories should use a second test before reporting a strain as susceptible, especially when testing strains isolated from invasive or serious infections. With the new (2007) CLSI breakpoints, the cefoxitin-disk test appears to be a good option for the detection of methicillin resistance in S. aureus. |
| Databáze: | Directory of Open Access Journals |
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