Optimization of Single Cell Protein Production by Candida utilis Using Juice Extracted from Pineapple Waste through Response Surface Methodology
| Autor: | Rosma, A., Liong, M. T., Mohd. Azemi, M. N., Wan Nadiah, W. A. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Malaysian Journal of Microbiology, Vol 1, Iss 1, Pp 18-24 (2005) |
| Druh dokumentu: | article |
| ISSN: | 1823-8262 2231-7538 |
| Popis: | Response surface methodology was applied to optimize protein content in Candida utilis grown in pineapple waste medium. A three-level full factorial design was used to develop a quantitative interpretation of mathematical models between the two variables studied, inoculum size 2.0-10.0% (v/v) and total soluble solids in medium (1-5 Brix) at 30 h fermentation time. Yeast cells were harvested, ruptured mechanically and the soluble extract was freeze-dried for determination of protein, vitamin-B, 5'-ribonucleotide and total sugar content. Maximum protein content in the yeast 66.61% (w/w) was obtained from the predicted optimum inoculum size of 7.83% (v/v) and Brix level of 3.02. Highest level of biomass, vitamin-B, 5'-ribonucleotide and total sugar content within the experimental region increased 216.8%, 17.5%, 38.0% and 60.8% respectively after optimization. A verification experiment, conducted at optimized protein content conditions produced values that were close to the predicted values, indicating the reliability of the model used. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|