Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits
Autor: | Saulo Tadeu Lemos Pinto Filho, Matheus Pippi da Rosa, Tiago Luis Eilers Treichel, Fabíola Dalmolin, Alencar Kolinski Machado, Francine Carla Cadoná, Ivana Beatrice Mânica da Cruz |
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Jazyk: | English<br />Portuguese |
Rok vydání: | 2018 |
Předmět: | |
Zdroj: | Semina: Ciências Agrárias, Vol 39, Iss 5, Pp 1993-2000 (2018) |
Druh dokumentu: | article |
ISSN: | 1676-546X 1679-0359 |
DOI: | 10.5433/1679-0359.2018v39n5p1993 |
Popis: | Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4. |
Databáze: | Directory of Open Access Journals |
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