Oxidative stress and viability of adipose tissue-derived mesenchymal cell cultures from the omentum of rabbits

Autor: Saulo Tadeu Lemos Pinto Filho, Matheus Pippi da Rosa, Tiago Luis Eilers Treichel, Fabíola Dalmolin, Alencar Kolinski Machado, Francine Carla Cadoná, Ivana Beatrice Mânica da Cruz
Jazyk: English<br />Portuguese
Rok vydání: 2018
Předmět:
Zdroj: Semina: Ciências Agrárias, Vol 39, Iss 5, Pp 1993-2000 (2018)
Druh dokumentu: article
ISSN: 1676-546X
1679-0359
DOI: 10.5433/1679-0359.2018v39n5p1993
Popis: Mesenchymal stem cells are a population of somatic cells found in several tissues of an adult organism, including adipose tissue. Reactive oxygen species (ROS) can cause cellular alterations, including mutagenesis and genomic instability and the development of diseases. Thus, it is important to understand ROS-induced damage to cell macromolecules such as DNA, proteins, and lipids. In this study, we investigated oxidative stress rates and viability of adipose tissue-derived mesenchymal stem cells (ADSCs) from the greater omentum of rabbits. Cell cultures were analyzed at different passages (1-5) using the dichlorofluorescein acetate assay for measuring ROS production and cell viability tests. ROS levels were highest at passage 2 and cell viability was highest at passage 4.
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