Designing of an ELISA Method for the Measurement of Serum Antibodies against Interferon-beta and the Study of the Frequency of these Antibodies in Patients with Multiple Sclerosis Treated with CinnoVex
| Autor: | Nasrin Zare, Sayyed Hamid Zarkesh Esfahani, Vahid Shaygannejad, Marjan Gharagozloo |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | مجله دانشکده پزشکی اصفهان, Vol 30, Iss 207, Pp 1481-1491 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1027-7595 1735-854X |
| Popis: | Background: Multiple sclerosis (MS) is a demyelinating disease of the central nervous system. Interferon-beta (IFN-β) was the first disease modifying drug to be approved for the treatment of MS. Some MS patients treated with IFN-β develop antibodies to the drug. Anti-IFN-β antibodies can reduce both bioactivity and clinical efficacy of IFN-β. The objective of this study was to develop an enzyme-linked immunosorbent assay (ELISA) method for the measurement of anti-IFN-β antibodies in MS patients treated with CinnoVex (a recombinant Interferon beta 1-a). Methods: An Indirect ELISA protocol was developed and validated for the measurement of anti-IFN-β antibodies. Sera were studied for anti-IFN-β antibodies from 40 healthy individuals and 42 MS patients treated with CinnoVex for at least 6 months. Findings: Patients were considered positive for Anti-IFN-β antibodies, if they had a positive sample with an optical density of more than 1.2. Anti-IFN-β antibodies were found in 12 MS patients treated with CinnoVex. 31.1% of MS patients treated with CinnoVex developed anti-IFN-β antibodies. Conclusion: ELISA is a simple, fast and inexpensive test for the assessment of anti-IFN-β antibodies in MS patients treated with IFN-β. Keywords: Multiple sclerosis, Interferon beta 1a, Antibodies, Enzyme-linked immunosorbent assay |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|