Gas Chromatography–Sensor System Aids Diagnosis of Inflammatory Bowel Disease, and Separates Crohn’s from Ulcerative Colitis, in Children

Autor: Rachael Slater, Kukatharmini Tharmaratnam, Salma Belnour, Marcus Karl-Heinz Auth, Rafeeq Muhammed, Christine Spray, Duolao Wang, Ben de Lacy Costello, Marta García-Fiñana, Stephen Allen, Chris Probert
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Sensors, Vol 24, Iss 15, p 5079 (2024)
Druh dokumentu: article
ISSN: 1424-8220
DOI: 10.3390/s24155079
Popis: The diagnosis of inflammatory bowel disease (IBD) in children and the need to distinguish between subtypes (Crohn’s disease (CD) and ulcerative colitis (UC)) requires lengthy investigative and invasive procedures. Non-invasive, rapid, and cost-effective tests to support these diagnoses are needed. Faecal volatile organic compounds (VOCs) are distinctive in IBD. VOC profiles can be rapidly determined using a gas chromatography–sensor device (OdoReader©). In an inception-cohort of children presenting with suspected IBD, we directly compared the diagnostic fidelity of faecal calprotectin (FCP, a non-specific protein marker of intestinal inflammation) with OdoReader© VOC profiles of children subsequently diagnosed with IBD with matched controls diagnosed with other gastrointestinal conditions. The OdoReader© was 82% (95% confidence interval 75–89%) sensitive and 71% (61–80%) specific but did not outperform FCP (sensitivity 93% (77–99%) and specificity 86% (67–96%); 250 µg/g FCP cut off) in the diagnosis of IBD from other gastrointestinal conditions when validated in a separate sample from the same cohort. However, unlike FCP and better than other similar technologies, the OdoReader© could distinguish paediatric CD from UC (up to 88% (82–93%) sensitivity and 80% (71–89%) specificity in the validation set) and justifies further validation in larger studies. A non-invasive test based on VOCs could help streamline and limit invasive investigations in children.
Databáze: Directory of Open Access Journals
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