| Autor: |
Ludmila Tavares Costa Ercolin, Gláucia Maria Bovi Ambrosano, Livia Fernandes Probst, Margarete C. Ribeiro-Dasilva, Scott L. Tomar, Rosana de Fátima Possobon |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Brazilian Journal of Oral Sciences, Vol 15, Iss 1 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1677-3225 |
| DOI: |
10.20396/bjos.v15i1.8647125 |
| Popis: |
Aim: To investigate the relation between uncooperative behavior and salivary cortisol level in children who underwent preventive dental care. Methods: The sample was composed by 10 children of both sexes aged 40 to 52 months, presenting uncooperative behavior during dental preventive treatments. The saliva collection was performed using a cotton wheel and an Eppendorf tube (Sarstedt Salivete®) in 3 different moments: a) at home, on a day without dental treatment and at the same time on the day of the sessions treatment; b) 30 min after the end of the session, when there was manifestation of uncooperative behavior; c) 30 min after the end of the session, when there was a cooperative behavior of the child. A sample of saliva was centrifuged for 5 minutes at 2400 rpm, 1 of mL of saliva was pipetted in an Eppendorf tube and stored in a freezer at -20 ° C. For the determination of the levels of salivary cortisol was used an Active® kit for cortisol enzyme immunoassay (EIA) DSL-10-67100, composed of specific rabbit antibody anti-cortisol. Data were analyzed statistically for the uncooperative behavior issued in the beginning and at the end of sessions, using the paired t test (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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