Molecular detection of Trypanosoma sp. and Blastocrithidia sp. (Trypanosomatidae) in phlebotomine sand flies (Psychodidae) in the Federal District of Brazil
| Autor: | Tauana de Sousa Ferreira, Thaís Tâmara Castro Minuzzi-Souza, Andrey José de Andrade, Thais Oliveira Coelho, Douglas de Almeida Rocha, Marcos Takashi Obara, Mariana Hecht, Nadjar Nitz, Rodrigo Gurgel-Gonçalves |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 6, Pp 776-779 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1678-9849 0037-8682 |
| DOI: | 10.1590/0037-8682-0076-2015 |
| Popis: | Abstract: INTRODUCTION : This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS : Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS : PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS : Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil. |
| Databáze: | Directory of Open Access Journals |
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