| Autor: |
Mohd. Rahil Hasan, Pradakshina Sharma, Saumitra Singh, Annu Mishra, Zaira Azmi, Jagriti Narang |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Biosensors and Bioelectronics: X, Vol 16, Iss , Pp 100431- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2590-1370 |
| DOI: |
10.1016/j.biosx.2023.100431 |
| Popis: |
The present study describes the creation of a 3D printed cassette named “3DP-PAC”, integrated to an electrochemical-aptasensor for the detection of dengue virus. It consists of an electrode cassette printed from a PLA non-conductive filament that provides a sophisticated design & support system to the delicate conductive paper-electrode. Chemically synthesized GO/ZnO-NC was used, which increases the sensor's sensitivity by accelerating the flow of electrons transferring. DENV DNA-Aptamer specifically binds to its target antigen of DENV, confirming its selectivity and demonstrating no cross reactivity with chikungunya virus antigen (CHIKV–Ag). The current study developed a 3D-based aptasensor for detecting dengue virus at a low level of detection (0.1 μg/ml). In human serum, the established platform performed well. This study paves the way for the manufacture of next-generation electrochemical biosensors utilizing 3D printing technology, with possible consequences for healthcare applications on the edge of commercialization. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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