Visualizing the ribonucleoprotein content of single bunyavirus virions reveals more efficient genome packaging in the arthropod host

Autor: Erick Bermúdez-Méndez, Eugene A. Katrukha, Cindy M. Spruit, Jeroen Kortekaas, Paul J. Wichgers Schreur
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Communications Biology, Vol 4, Iss 1, Pp 1-13 (2021)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-021-01821-y
Popis: Bermúdez-Méndez et al. describe a FISH-immunofluorescence method that allows simultaneous visualization of bunyavirus genome segments and virions at single-molecule resolution. They show that the packaging of bunyavirus genome segments into virions is a flexible, non-selective process and that genome packaging is more efficient in insect cells compared to mammalian cells.
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