| Autor: |
Staršíchová Andrea, Kubala Lukáš, Lincová Eva, Pernicová Zuzana, Kozubík Alois, Souček Karel |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Biological Procedures Online, Vol 11, Iss 1, p 9017 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1480-9222 |
| DOI: |
10.1007/s12575-009-9017-9 |
| Popis: |
Abstract The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic of progression of serious pathological states such as cancer and fibrosis. In this study, we report on the application of a real-time noninvasive system for dynamic monitoring of cellular plasticity. Analysis of the cell impedance profile recorded as cell index using a real-time cell analyzer revealed its significant increase after the treatment of prostate epithelial cells with the transforming growth factor-β1. Changes in the cell index profile were paralleled with cytoskeleton rebuilding and induction of epithelial–mesenchymal transition and negatively correlated with cell proliferation. This novel application of such approach demonstrated a great potential of the impedance-based system for noninvasive and real-time monitoring of cellular fate. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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