Prognostic Value of Cyclin-D1 Expression in Head-a nd-Neck Squamous Cell Carcinoma: A Half-Decade Follow-Up
| Autor: | Azka Haroon, Muhammad Nadeem Zafar, Nighat Ara, Saadia Muneer, Zunaira Saeed, Zainab Asif Sukhera |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Islamic International Medical College, Vol 18, Iss 1, Pp 22-26 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1815-4018 2410-5422 |
| Popis: | Objective: To check the expression pattern of cyclin-D1 in stage I head and neck squamous cell carcinoma (H.N.S.C.C.) cases (evaluated based on history, clinical, medical, and radiological records) in our setting and evaluate the role of cyclin-D1 as a prognostic marker of H.N.S.C.C. (considering recurrence within five years and metastasis). Study Design: Retrospective cohort study. Place and Duration of Study: The study was carried out at Armed Forces Institute of Pathology (A.F.I.P.)Rawalpindi working in conjunction with the Department of Radiation Oncology, Rawalpindi. The duration of study was from 7 March 2022-14 October 2022. Materials and Methods: The study comprised 92 patients from year 2015-16 of stage I diagnosed with squamous cell carcinoma of head and neck with complete five-year follow-up after fulfilling the inclusion and exclusion criteria. After collecting and matching the data on follow-up with record of the patients from Department of Radiation Oncology, C.M.H., Rawalpindi; paraffin-embedded blocks of these patients from year 2015/2016 were retrieved. Fresh Hematoxylin and Eosin (H&E) slides were prepared, and diagnosis was reconfirmed, vimentin stain was applied to check the antigenicity of the tissue followed by application of cyclin-D1 immuno-histochemical marker (I.H.C.) marker with controls to check the expression pattern. Results: In our study we included a total of 92 diagnosed cases of stage I H.N.S.C.C. with a complete 5-year follow-up. Out of 92 cases male to female ratio was almost 3:1 with males comprising a total of 68 (73.9%) and females comprising 24 (26.1%). Mean age of patients was 62.48+ 11.439 years. Larynx was the most frequently involved site 34.8% (n=32), followed by tongue 23.9% (n=22) and buccal mucosa 13.0% (n=12). Cyclin-D1expression and tumor grade were revealed to have a statistically significant clinicopathological relationship (p value 0.001). A statistically significant association between the expression of cyclin-D1 and tumor recurrence (p value < 0.001) and duration of tumor recurrence (p value 0.003) was seen. The cyclin-D1 expression was also compared with metastasis which was also statistically significant (p value 0.003). Conclusion: An increased risk of metastasis and recurrence is linked to increased levels of cyclin-D1 expression in the early years. Hence it is valuable to include cyclin-D1 expression in the initial diagnostic work up of H.N.S.C.C. for an early prognostic assessment. Further use of these results could be made for targeted treatment of head and neck malignancies. |
| Databáze: | Directory of Open Access Journals |
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