| Autor: |
Marina Shenkman, Navit Ogen-Shtern, Gerardo Lederkremer |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Bio-Protocol, Vol 7, Iss 14 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2331-8325 |
| DOI: |
10.21769/BioProtoc.2393 |
| Popis: |
Modifications of N-linked oligosaccharides of glycoproteins soon after their biosynthesis correlate to glycoprotein folding status. These alterations can be detected in a sensitive way by pulse-chase analysis of [2-3H]mannose-labeled glycoproteins, with enzymatic removal of labeled N-glycans, separation according to size by HPLC and radioactive detection in a scintillation counter. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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