Comparative analysis of human sperm glycocalyx from different freezability ejaculates by lectin microarray and identification of ABA as sperm freezability biomarker

Autor: Ai-jie Xin, Yan-cheng Wu, Hui Lu, Li Cheng, Yi-hua Gu, Hua Diao, Guo-wu Chen, Bin Wu, Zheng Li, Sheng-ce Tao, Xiao-xi Sun, Hui-juan Shi
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Clinical Proteomics, Vol 15, Iss 1, Pp 1-8 (2018)
Druh dokumentu: article
ISSN: 1542-6416
1559-0275
DOI: 10.1186/s12014-018-9195-z
Popis: Abstract Background Semen cryopreservation has been widely applied in assisted reproductive technologies and sperm bank, but it causes considerable impairments on sperm quality. It is necessary to find an evaluation indicator for determining the sperm-freezing tolerance. Methods The glycocalyx of good freezability ejaculates was compared with poor freezability ejaculates by lectin microarray. The significant different lectins were validated by flow cytometry (FACS). To analyze the relationship between the potential biomarker and the tolerance of sperm to cryopreservation, 60 samples with different recovery rates were collected and detected the lectin-binding intensity by FACS. The receiver operating characteristic (ROC) curve was analyzed to test the capability of the lectin as a potential biomarker for detecting the sperm freezablility. Results ABA and DSL were found to develop significant differences between them. Further validation showed that ABA was significantly negative correlated with the sperm recovery rates (r = − 0.618, P
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