RT-PCR (Reverse Transcription - Polymerase Chain Reaction): Suatu Cara Pendeteksi Perubahan-Perubahan Ekspresi Gen pada Penyakit
| Autor: | Elza Ibrahim Auerkari, Hari Sunarto, Ariadna Djais |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Dentistry Indonesia, Vol 5, Iss 3, Pp 162-165 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1693-9697 2355-4800 |
| DOI: | 10.14693/jdi.v5i3.781 |
| Popis: | The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very small amount of specific mRNA to obtain cDNA clones and to detect mRNA transcripts from abnormal cells in a background of normal cells. |
| Databáze: | Directory of Open Access Journals |
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