Biosynthesis of antioxidative enzymes and polyphenolics content in calli cultures of Prunella vulgaris L. in response to auxins and cytokinins

Autor: Hina Fazal, Bilal Haider Abbasi, Nisar Ahmad, Bushra Noureen, Jahangir Shah, Dan Ma, Liu Chuanliang, Fazal Akbar, Muhammad Nazir Uddin, Haji Khan, Mohammad Ali
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Artificial Cells, Nanomedicine, and Biotechnology, Vol 48, Iss 1, Pp 893-902 (2020)
Druh dokumentu: article
ISSN: 21691401
2169-141X
2169-1401
DOI: 10.1080/21691401.2020.1771349
Popis: Prunella vulgaris L. is one of the therapeutic herbs containing various polyphenolics, which is used for multiple medicinal purposes. In this study, plant growth regulators (PGRs)-induced calli cultures from seed-derived leaf explants were exploited for the production of stress enzymes and polyphenolics. A growth curve was plotted for each PGR for 49 days period, which showed a distinct lag, log and decline phases. Here, the combination of naphthalene acetic acid (NAA) and 6-benzyleadenine (BA; 0.5 and 2.0 mg l−1) produced maximum fresh (6.32 FW-g/100 ml) and dry biomass (0.75 DW-g/100 ml) in contrast to control. The maximum synthesis of SOD (0.0154 FW-nM/min/mg) was detected on media comprising mixture of NAA and BA (1.5 mg l−1), while POD enzyme (0.366 FW-nM/min/mg) was higher at 0.5 mg l−1 NAA and 2, 4-dichlorophenoxy acetic acid. Further, NAA and BA (1.5 and 2.0 mg l−1) boosted up the synthesis of phenolics (18.83 GAE-mg/g-DW) and flavonoids content (18.05 RE-mg/g-DW) than control. Moreover, NAA of 1.0 and 2.0 mg l−1 were found supportive for maximum antioxidant activity (87.4%) and total protein (716 µg BSAE/mg-DW). This study will contribute in the development of cell culture in fermenter and synthesis of antioxidant secondary metabolites for commercial uses.
Databáze: Directory of Open Access Journals
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