Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography
| Autor: | Daniel Rinaldo, Marcelo Aparecido Silva, Clenilson Martins Rodrigues, Tamara Regina Calvo, Miriam Sannomiya, Lourdes Campaner dos Santos, Wagner Vilegas, Hélio Kushima, Clélia Akiko Hiruma-Lima, Alba Regina Monteiro de Souza Brito |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 29, Iss 5, Pp 947-949 (2006) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422006000500011 |
| Popis: | High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses. |
| Databáze: | Directory of Open Access Journals |
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