| Popis: |
The present work sought to implement a model different from the more traditional ones for the fermentation process of ethanol production by the action of the fungus Saccharomyces cerevisiae, using a relevant metabolic network based on the glycolytic Embden–Meyerhof–Parnas route, also called “EMP”. We developed two models to represent this phenomenon. In the first model, we used the simple and unbranched EMP route, with a constant concentration of microorganisms throughout the process and glucose as the whole substrate. We called this first model “SR”, regarding the Portuguese name “sem ramificações”, which means “no branches”. We developed the second model by simply adding some branches to the SR model. We called this model “CR”, regarding the Portuguese name “com ramificações”, which means “with branches”. Both models were implemented in MATLABTM software considering a constant temperature equal to 32 °C, similar to that practiced in sugar and ethanol plants, and a wide range of substrate concentrations, ranging from 30 to 100 g/L, and all the enzymes necessary for fermentation were already expressed in the cells so all the enzymes showed a constant concentration throughout the fermentation. The addition of common branches to the EMP route resulted in a considerable improvement in the results, especially predicting ethanol production closer to what we saw experimentally. Therefore, the results obtained are promising, making adjustments consistent with experimental data, meaning that all the models proposed here are a good basis for the development of future metabolic models of discontinuous fermentative processes. |
