| Autor: |
Antov Mirjana G., Šćiban Marina B., Adamović Slavica R., Klašnja Mile T. |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Acta Periodica Technologica, Vol 2007, Iss 38, Pp 3-10 (2007) |
| Druh dokumentu: |
article |
| ISSN: |
1450-7188 |
| DOI: |
10.2298/APT0738003A |
| Popis: |
Investigation of an extraction procedure of protein coagulants from common bean seed regarding concentration of NaCl and pH was performed. High values of protein concentration and coagulation activity in crude extract (9.19 g/l and 23.9%, respectively) were obtained when the extraction was performed using 0.5 mol/l NaCl and water as solvent, which represents an advantage for economic and environmental reasons. Crude extract of common bean seed was purified by precipitation at two different percentages of (NH4)2SO4 saturation, followed by batch ion-exchange chromatography. The highest obtained coagulation activity, 45%, was determined in fraction that was eluated at 1.75 mol/l NaCl from resin loaded with proteins precipitated upon 80-100% (NH4)2SO4 saturation. High values of coagulation activity showed by some eluates suggest their application as natural coagulant for water purification. . |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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