| Autor: |
L. SJÖSTROM, P. BJÖRNTORP, J. VRÁNA |
| Jazyk: |
angličtina |
| Rok vydání: |
1971 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 12, Iss 5, Pp 521-530 (1971) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)39470-0 |
| Popis: |
Diameters of fat cells in adipose tissue slices, floating in an isoosmolar solution, were measured under a microscope. The slices were obtained from percutaneous biopsies by freeze-cutting after brief formaldehyde fixation. All cells in a given part of the slice were measured, thus avoiding selection. A normal distribution of fat cell diameters could be demonstrated with this method, as has been found with previously described methods. The error of the method was 2.6% for diameter and 8.0% for weight determinations. Storage of adipose tissue at 4°C for 48 hr had no effects on cell size determinations.Results with this micrcscopic method were compared with those obtained from a previously described method for automatic determinations of osmium-fixed fat cells. The latter method was slightly modified by using a viscous electrolyte, which prevented sedimentation of large fat cells, and by using sonication to complete cell separation. The methods agreed closely.A method for calculating mean fat cell weight using osmium-fixed fat cells is described, which makes determinations of sample wet weight and ratio of lipid to wet weight unnecessary. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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