Antibiotic resistance pattern and virulence genes in avian pathogenic Escherichia coli (APEC) from different breeding systems

Autor: Elisa Sgariglia, Nicholas Aconiti Mandolini, Maira Napoleoni, Laura Medici, Roberta Fraticelli, Michela Conquista, Paola Gianfelici, Monica Staffolani, Stefano Fisichella, Marinella Capuccella, Marta Sargenti, Gianni Perugini
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Veterinaria Italiana, Vol 55, Iss 1 (2019)
Druh dokumentu: article
ISSN: 0505-401X
1828-1427
DOI: 10.12834/VetIt.1617.8701.1
Popis: Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are the main weapon to reduce incidence and mortality associated to it. However, indiscriminate use of antibiotics may lead to therapy failure and economic losses for the breeder. The aims of this study were to, determine the antibiotic resistance of Escherichia coli isolates, evaluate the correlation between E. coli isolation and systems of breeding included in this study, and identify the avian pathogenic E.coli (APEC) amongst the E. coli strains isolated. A total of 51 E. coli strains were subjected to antimicrobial susceptibility test and they were screened for the presence of virulence genes through PCR. Resistance was most frequently detected against ampicillin and nalidixic acid meanwhile E. coli isolates showed less resistance to the cephalosporins. Overall, 40% of the isolates showed resistance to at least three or more antimicrobials and 16/51 isolates were defined APEC strains. The virulence genes iucD, cvi/cva, irp2 and iss were detected from all 16 APEC strains. The virulence genes tsh, vat, papC, and astA were detected from 11, 7, 5 and 3 APEC strains, respectively. Results demonstrated the importance of studies on APEC and antibiotic resistance genes in Italy, and it was shown that the systems of breeding might influence the antibiotic resistance.
Databáze: Directory of Open Access Journals
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