| Autor: |
Junya Ichikawa, Ryuji X Yamada, Rieko Muramatsu, Yuji Ikegaya, Norio Matsuki, Ryuta Koyama |
| Jazyk: |
angličtina |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Journal of Pharmacological Sciences, Vol 104, Iss 4, Pp 387-391 (2007) |
| Druh dokumentu: |
article |
| ISSN: |
1347-8613 |
| DOI: |
10.1254/jphs.SC0070162 |
| Popis: |
Although primary cultures of neurons are essential methods for cell biological and pharmacological researches, many animals must be sacrificed for each experiment. Here we introduce a novel system to cryopreserve hippocampal granule cells (GCs) prepared from postnatal rats. Being thawed after as long as 60 days of cryopreservation, GCs expressed the mature neuronal marker MAP-2 and elongated single tau-1-positive axons and multiple tau-1-negative dendrites. These properties closely resembled intact GCs in primary cultures, providing the advantage of being able to repeatedly prepare stable cultures with a single sacrifice of animals. Keywords:: dentate gyrus, primary culture, Prox1 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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