Genotype-dependent development of cellular and humoral immunity in the spleen and cecal tonsils of chickens stimulated in ovo with bioactive compounds

Autor: J.P. Madej, J. Skonieczna, M. Siwek, A. Kowalczyk, E. Łukaszewicz, A. Slawinska
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Poultry Science, Vol 99, Iss 9, Pp 4343-4350 (2020)
Druh dokumentu: article
ISSN: 0032-5791
DOI: 10.1016/j.psj.2020.05.048
Popis: Prebiotics, probiotics, and synbiotics, delivered in ovo influence the colonization and development of the peripheral immune system in poultry. This study aimed to investigate the influence of the host genotype (broiler chickens [Ross 308] and old native Polish breed Green-legged Partridgelike [GP] chickens) on the number of B and T cells in the spleen and cecal tonsils (CT). The solution of a bioactive compound was injected in ovo on day 12 of egg incubation: prebiotics (galactooligosaccharides [GOS]), probiotics (Lactococcus lactis subsp. cremoris IBB477), and synbiotics (GOS + L. lactis). The samples were collected on day 7, day 21, and day 42 after hatching (n = 8). The number of Bu-1+ (B) cells, CD4+ cells, and CD8+ cells in the spleen and CT was estimated using immunohistochemistry. The number of germinal centers (GC) was determined in the spleen. In broilers, probiotics increased (P
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