| Autor: |
Luanna Munhoz Zabaglia, Mariane Avante Ferraz, Weendelly Nayara Pereira, Wilson Aparecido Orcini, Roger Willian de Labio, Agostinho Caleman Neto, Fernanda Wisnieski, Juliana Garcia de Oliveira, Marilia de Arruda Cardoso Smith, Spencer Luiz Marques Payão, Lucas Trevizani Rasmussen |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1678-9199 |
| DOI: |
10.1186/s40409-015-0054-3 |
| Popis: |
Abstract Background Tumor necrosis factor plays a critical role in the pathogenesis of gastric diseases such as gastric cancer, and an abnormal inflammatory response has frequently been observed in dyspeptic patients. Helicobacter pylori infection can induce a gastric mucosal inflammatory response that may be influenced by -308 (G > A) polymorphisms and gene expression of theTNF-α gene. Methods One hundred and thirty-four gastric biopsy samples were collected from patients of both genders (61♂ and 73♀, mean age 40.3 ± 24.2 years) with gastric symptoms. The -308 (G > A) polymorphism of TNF-α was characterized using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). The expression level was measured using real-time PCR, and relative quantification (RQ) was calculated using the comparative CT method (2-ΔΔCT). Results The analysis revealed an increase in TNF-α gene expression in patients with gastritis; on the other hand, no statistical differences were observed in patients with gastric cancer. In addition, no association was found among -308 polymorphism genotypes, virulence markers, or TNF-α gene expression. Conclusions Helicobacter pylori induces a large increase in TNF-α expression in patients with gastritis, regardless of tissue inflammation, but after the tissue becomes neoplastic, the presence of bacteria did not influence expression. These results suggest that the TNF-α pathway may play an important role in the progression from gastritis to gastric cancer |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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